Journal: Journal of cellular physiology

Article Title: Stress-Induced Activation of Apoptosis Signal-Regulating Kinase 1 Promotes Osteoarthritis

doi: 10.1002/jcp.25186

Figure Lengend Snippet: ASK1 KO mouse protected against OA changes after partial meniscectomy. (A) H&E and alcian blue (proteoglycan) stained sections of WT, Het, and KO mice after surgery show articular cartilage changes relative to sham limbs. Het and KO mice show less severe degradation, cartilage loss, and fibrosis than WT (compare areas marked with yellow arrows). (B) Box and whisker plot showing OARSI OA scores for representative WT, Het and KO mouse limbs. (C,D) MicroCT reconstruction of WT, Het, and KO knee joints after meniscectomy surgery showed a thinning in subchondral bone in WT, concurrent with early stages of OA development, which is absent in Het and KO mouse knees (white arrows). (n = 7; * = P ≤ 0.05; ** = P ≤ 0.01; scale bars = 1mm).

Article Snippet: Micro-computed tomography (MicroCT) analysis Each knee joint was harvested at the end of the experimental protocol, fixed in 4% paraformaldehyde, and subjected to microCT analysis (Scanco μCT 40; Basserdorf, Switzerland).

Techniques: Staining, Whisker Assay

Journal: Journal of cellular physiology

Article Title: Stress-Induced Activation of Apoptosis Signal-Regulating Kinase 1 Promotes Osteoarthritis

doi: 10.1002/jcp.25186

Figure Lengend Snippet: ASK1 KO mice are protected against OA 8 weeks after cartilage injury. (A,B) H&E and Alcian blue stained sections of WT and KO mice after surgery showed articular cartilage degradation compared to sham surgeries. Compared to WT, KO knees have thicker articular cartilage, smoother architecture, and less fibrosis, inflammatory infiltration and subchondral bone thickening (compare areas marked with yellow arrows). (C) Magnified views of WT and KO limbs showing areas of chondrocyte hypertrophy, osteophyte formation, and proteoglycan loss (insets). (D) Box and whisker plot showing OARSI OA scores for representative WT and KO mouse limbs. (E) MicroCT reconstruction of WT and KO limbs show an increase in subchondral bone thickness (BV/TV) in WT, but this effect is abrogated in KO. (n = 3; * = P ≤ 0.05; ** = P ≤ 0.01; # = P ≤ 0.05 compared to unoperated; scale bars = 500 μm).

Article Snippet: Micro-computed tomography (MicroCT) analysis Each knee joint was harvested at the end of the experimental protocol, fixed in 4% paraformaldehyde, and subjected to microCT analysis (Scanco μCT 40; Basserdorf, Switzerland).

Techniques: Staining, Whisker Assay

Journal: Journal of cellular physiology

Article Title: Stress-Induced Activation of Apoptosis Signal-Regulating Kinase 1 Promotes Osteoarthritis

doi: 10.1002/jcp.25186

Figure Lengend Snippet: Characterization of WT and KO mice articular cartilage during aging. (A) H&E and Alcian blue (proteoglycan) stained sections of WT and KO mice showed comparatively similar articular cartilage of the knee joint at 2 months and 1 year, but 2 year old WT mice showed increased articular cartilage wear and less proteoglycan, compared to the cartilage thickness observed in the ASK1 KO mouse (compare areas marked with yellow arrows). (scale bar = 500 μm) (B) Box plot showing OARSI OA scores for representative WT and KO mouse limbs. (C,D) MicroCT reconstruction of WT and KO hind limb joints at 2 months, 6 months, 1 year, and 2 years showed a decrease in subchondral bone thickness (BV/TV) compared to WT (arrows). (** = P ≤ 0.01 compared to WT; # P ≤ 0.05 compared to 2mo; ## P ≤ 0.01 compared to 2mo; n = 3/age; Scale bar = 1 mm).

Article Snippet: Micro-computed tomography (MicroCT) analysis Each knee joint was harvested at the end of the experimental protocol, fixed in 4% paraformaldehyde, and subjected to microCT analysis (Scanco μCT 40; Basserdorf, Switzerland).

Techniques: Staining